Fluorescence molecular relaxation studies of protein dynamics

Molecular dynamics studies of NMR relaxation in proteins.

There is a growing awareness that the visualization of proteins as large rigid molecules, each of which can be described by a single static structure, is inadequate. Instead, it is now realized that proteins undergo structural fluctuations which span a range of times from subpicoseconds to milliseconds or longer. In this report we are concerned with the relation between protein fluctuations occ...

Single-molecule fluorescence studies of protein folding and conformational dynamics.

Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones

Folding of newly synthesized proteins is an essential part of protein biosynthesis and misfolding can result in protein aggregation which can also lead to several severe diseases. Protein folding is a highly heterogeneous process and rarely populated intermediate states may play an important role. Single-molecule techniques are ideally suited to resolve these heterogeneities. In this thesis, I ...

Comparative Investigation of R213G Mutation in DNA-Binding Domain of P53 Protein via Molecular Dynamics Simulation

Introduction: P53 is a tumor suppressor protein with numerous missense mutations identified in its gene. These mutations are observed in a vast number of cancers. R213G is one of them which has a role in metastatic lung cancers. In this research, R213G was studied in comparison with the wild type via molecular dynamics simulation. Method: For the three-dimensional structure of the wild-type P53...

Lipid hydration and mobility: an interplay between fluorescence solvent relaxation experiments and molecular dynamics simulations.

Fluorescence solvent relaxation experiments are based on the characterization of time-dependent shifts in the fluorescence emission of a chromophore, yielding polarity and viscosity information about the chromophore's immediate environment. A chromophore applied to a phospholipid bilayer at a well-defined location (with respect to the z-axis of the bilayer) allows monitoring of the hydration an...